Evaluation of dual p16/Ki-67 immunostaining on anal cytology specimens.

INTRODUCTION: Dual immunostaining for p16/Ki67 is FDA-approved for use on liquid-based cervical cytology specimens; however, the utility of dual staining in anal cytology especially for ASCUS risk stratification is not well established. METHODS: We investigated dual staining performance on anal cytology specimens and correlated with subsequent cytologic interpretation, high-risk HPV status, and anal biopsy results. Dual staining for p16/Ki-67 was performed on all liquid-based anal cytology specimens from December 2021 to June 2022 (n = 43). RESULTS: Three patients had high grade squamous intraepithelial lesion (HSIL/AIN2-3) on biopsy; dual staining was positive in all three cases. All HR-HPV negative cases were negative for dual staining. Among the 12 ASCUS samples with subsequent anal biopsy results all also had HR-HPV testing. Due to small sample size of cases with squamous intraepithelial lesion (SIL) diagnosed on biopsy, the sensitivity and positive predictive value was not calculated. However, the specificity and negative predictive value of p16/Ki-67 dual staining for SIL of any grade on biopsy were 1 (95% CI: 0.66-1) and 0.9 (95% CI: 0.65-0.97) respectively, whereas the specificity and negative predictive value of HR-HPV testing for SIL of any grade on biopsy were 0.44 (95% CI: 0.14-0.79) and 0.8 (95% CI: 0.41-0.96) respectively. CONCLUSION: Dual p16/Ki-67 staining indicates transforming HPV infection and could help serve as an ancillary test for risk stratification for atypical anal cytology specimens. Among ASCUS samples, dual staining was specific for SIL of any grade with a high negative predictive value and therefore could be useful in clinical practices with limited availability for follow-up care.

The significance of ASC-H and LSIL dual interpretation with risk stratification: one institution experience.

INTRODUCTION: The 2014 Bethesda System categorizes squamous lesions as low-grade squamous intraepithelial lesions (LSIL) and high-grade squamous intraepithelial lesions (HSIL). It also includes intermediate morphologic terminology, such as atypical squamous cells of undetermined significance (ASC-US) and atypical squamous cells, cannot rule out a high grade squamous intraepithelial lesion (ASC-H). Consensus is lacking if when ASC-H is present in an unequivocal LSIL (LSIL + ASC-H) versus ASC-H alone predicts a neoplasm with a different biologic behavior and which is its association with high-risk human papillomavirus (HPV). MATERIALS AND METHODS: We reviewed the Columbia University Medical Center Pathology department patient's database from October 2012 through December 2014 and found 2498 cytology samples of LSIL, ASC-H, HSIL, and LSIL + ASC-H with both follow-up histologic samples and HPV tests by Roche cobas. Our objective was to identify, if any, differences in biologic behavior and HPV status present in LSIL + ASC-H compared with ASC-H and other lesions. RESULTS: CIN2+ was documented in tissue examination in 102 from 311 LSIL + ASC-H (32.8%), 101 from 219 ASC-H (46.1%), 252 from 326 HSIL+ (77.3%), and 150 from 1642 LSIL (9.08%). HPV distribution shows significant differences between all diagnostic categories. CONCLUSIONS: LSIL + ASC-H appears to have a distinctive HPV distribution pattern that clearly differs from ASC-H and LSIL and approaches HSIL; however, the predictive value for CIN2+ appears higher for ASC-H than LSIL + ASC-H. Our literature review identified conflicting findings, probably suggesting a lack of reproducibility in cytologic criteria and the need for consistent inclusion of ASC-H and LSIL when both are present.

Negative Roche cobas HPV testing in cases of biopsy-proven invasive cervical carcinoma, compared with Hybrid Capture 2 and liquid-based cytology.

INTRODUCTION: The objective of this study was to conduct a retrospective analysis of results of cytology and Roche cobas (RC) and Hybrid Capture 2 (HC2) human papillomavirus (HPV) screening tests in cases of biopsy-proven invasive cervical carcinoma. MATERIALS AND METHODS: The clinical data were obtained at a university hospital in New York, NY, between 2004 and 2017. Results of cytology, reported per Bethesda classification system, and HPV screening in 177 identified cases with cytology and biopsy-proven diagnosis of cervical carcinoma were included in the analysis. RESULTS: Two cohorts were analyzed. Of the 177 identified cases, cotesting was performed for 100 patients. Among these 100, cotesting screening results would not trigger immediate colposcopy in 6%; HPV screening results were reported as negative in 16% (16% of all RC, 16% of all HC2, 16% total) and, if HPV was the only screening modality, would not trigger a colposcopy. Of the 177 total cases, 128 patients underwent cytology screening prior to biopsy, with a cytology diagnosis that, alone, would not trigger immediate colposcopy in 14%. CONCLUSIONS: The HPV DNA screening and cytology screening alone were negative for 16% and 14%, respectively, of patients with biopsy-proven diagnoses of invasive carcinoma of cervical origin, without a significant difference in failure rates between cytology, HC2, and RC. The cotesting approach had a significantly lower failure rate (6%) compared with the 2 other screening modalities alone.

Cytologic features of needle aspiration of ovarian sex cord tumor with annular tubules: Report of two cases and literature review.

Sex cord tumor with annular tubules (SCTAT) is a rare ovarian tumor with characteristic microscopic morphologic features. Diagnosis most often is based on examination of tissue specimens. Cytologic features of this tumor rarely have been described in the English literature. Herein, we report cytologic findings of cyst aspiration fluid in two cases of SCTAT, with cyto-histologic correlation.

Tumores de la glándula parótida: experiencia en 165 pacientes / Parotid gland tumors: experience in 165 patients

Se presenta una serie de 165 pacientes con tumores de parótida, tratados quirúrgicamente. La utilidad de la punción citológica resultó evidente, como también la de la biopsia por congelación. La extensión de la cirugía debe ser oncológicamente amplia. La radioterapia postoperatoria demostró eficacia

Tumores de la glándula parótida: experiencia en 165 pacientes / Parotid gland tumors: experience in 165 patients

Se presenta una serie de 165 pacientes con tumores de parótida, tratados quirúrgicamente. La utilidad de la punción citológica resultó evidente, como también la de la biopsia por congelación. La extensión de la cirugía debe ser oncológicamente amplia. La radioterapia postoperatoria demostró eficacia (AU)

Variante gemini del colgajo miocutaneo del pectoral mayor / Variant \"gemini\" of pectoral major miocutaneous flaps

Se presenta la experiencia obtenida en 8 casos con la correción simultánea de defectos de piel y mucosa mediante el empleo de una modificación del colgajo miocutáneo del pectoral mayor en doble isla "gemini". Se señalan los resultados en general satisfactorios, así como su particular indicación en la reparación de lesiones del sector anterior de la cavidad oral

Variante gemini del colgajo miocutaneo del pectoral mayor / Variant "gemini" of pectoral major miocutaneous flaps

Se presenta la experiencia obtenida en 8 casos con la correción simultánea de defectos de piel y mucosa mediante el empleo de una modificación del colgajo miocutáneo del pectoral mayor en doble isla "gemini". Se señalan los resultados en general satisfactorios, así como su particular indicación en la reparación de lesiones del sector anterior de la cavidad oral (AU)

Cáncer de pene: análisis estadístico / Penile neoplasms: statistical analysis

En el presente estudio se hace un análisis estadístico de la información obtenida de los expedientes de los pacientes con cáncer de pene diagnosticados en el Centro Médico de Mayaguez entre los años 1979 a 1984. Estos resultados se comparan con publicaciones anteriores. Se hace una exhortación de educación a la comunidad para prevenir esta malignidad

Carcinoma de labio. Experiencia en 300 casos. / Lip carcinoma. Experience in 300 cases

Se analiza la experiencia recogida entre 1969 y 1982 en 300 pacientes portadores de carcinomas de labio. Se revisan las caracteristicas de la poblacion portadora y las del tumor, referidas a su localizacion, en forma macroscopica, variedad histologica y estadio. Los autores exponen los tratamientos efectuados en el tumor primario y en las adenopatias y hacen consideraciones sobre sus indicaciones. Evaluan los resultados obtenidos en cuanto a sobrevida global y a la correspondiente a los distintos estadios, procedimientos terapeuticos, existencia de adenopatias metastasicas y procedimientos previos no exitosos. Insisten sobre la importancia del diagnostico temprano y el tratamiento correcto como precondiciones para la obtencion de altas tasas de curacion

Carcinoma de labio. Experiencia en 300 casos. / Lip carcinoma. Experience in 300 cases

Se analiza la experiencia recogida entre 1969 y 1982 en 300 pacientes portadores de carcinomas de labio. Se revisan las caracteristicas de la poblacion portadora y las del tumor, referidas a su localizacion, en forma macroscopica, variedad histologica y estadio. Los autores exponen los tratamientos efectuados en el tumor primario y en las adenopatias y hacen consideraciones sobre sus indicaciones. Evaluan los resultados obtenidos en cuanto a sobrevida global y a la correspondiente a los distintos estadios, procedimientos terapeuticos, existencia de adenopatias metastasicas y procedimientos previos no exitosos. Insisten sobre la importancia del diagnostico temprano y el tratamiento correcto como precondiciones para la obtencion de altas tasas de curacion